facebook    twitter    linkedin
Bacterial Cell Protein Expression Services

Bacterial Cell Protein Expression Services

Recombinant protein production is a promising field and there seems no sign that its expansion will abate anytime soon. Bacterial expression system is often the first choice for lab research and initial development in commercial activities or as a useful benchmark for comparison among various expression platforms. This system can satisfy researchers’ desire to understand the functions of hundreds and thousands of proteins. Meanwhile, it has the characteristics of simple culture, short growth cycle and low cost.

Bacterial-Cell-Protein-Expression-Services

Creative BioMart has comprehensively selected easy-to-handle tools for efficient gene expression based on different well-developed microorganisms: E. coli, Bacillus Subtilis, Bacillus Megaterium and Lactococcus Lactis.

E. coli Expression System

Click Here to See How to Select An Appropriate Protein Expression System→

System Description

E. coli expression system is the best developed bacterial expression system. It exhibits the advantages of

  • Clear genetic background
  • Easy to manipulate
  • Short cell proliferation time, short expression period
  • Easily to be purified from inclusion bodies
  • Well-established labeling protocols
  • Can be scaled up through fermentation
  • Minimal culturing cost

Bacillus subtilis Expression System

System Description

Bacillus subtilis has become a popular bacterial host for industrial and pharmaceutical protein production presently. Creative BioMart’s Bacillus subtilis system is competent for high-level monomeric protein expression. Additionally, it also shows the features including:

  • Tightly controlled expression system with a long history
  • Suitable for both intracellular and extracellular expression
  • Do not need expensive inducers like IPTG
  • Less chance of protein aggregation
  • Suitable for metabolic engineering gene expression
  • Shuttle vectors with different tags (His-tag, Strep-tag) for both E. coli and B. subtilis
  • Endotoxin-free
  • Potentially useful for cloning of genes with toxic product
  • Easy transformation

Bacillus Megaterium Protein Expression System

System Features Description

  • Suitable for both intracellular and extracellular, small-scale and large-scale protein production
  • Can be used to establish an inducible system due to the xylA promoter
  • Can be used to establish a constitutive expression system
  • Alkaline protease activity-free and endotoxins-free
  • Vectors are high performance, there are two ribosome binding sites (2RBS) for simultaneous dual expressions
  • Support versatile purification (6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Vectors with removable purification tag
  • Protoplasts specifically optimized for transformation

Lactococcus Lactis Expression System

System Features Description

In recent years, Lactococcus lactis has become one of the most important Gram-positive bacterial expression systems. Compared with Escherichia coli, it has the advantages of no gras and no endotoxin, so it is very important for treatment and food application. As gram-positive, the potential for extracellular production is advantageous.

  • Stable cell line with promising productivity
  • Easy to be modified
  • Suitable for metabolite engineering
  • Suitable for both intracellular and extracellular expressions
  • Can be used as a food-grade expression system
  • Can be used to establish inducible and constitutive expression systems
  • Endotoxin-free
  • Few chances to form inclusion bodies
  • Reduced protease activity
  • Suitable for expression of genes with toxic products
  • Suitable for expression of membrane proteins

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.

CONTACT OUR TEAM