Cell surface display is the technology that uses genetic engineering to fuse foreign protein or peptide with an anchor motif, so as to fix the target fragment on the cell surface. The effectiveness of this technology has been confirmed in many fields of protein engineering. At present, cell surface display technology has been applied in many different biotechnology and biomedical fields, such as vaccine development, bioabsorbent, biocatalyst, biosensor, etc.
Creative BioMart has established advanced technology and a complete platform in the field of protein synthesis and biotechnology after decades of development. We have built a variety of protein expression platforms based on eukaryotic and prokaryotic cells (mammalian, bacterial, yeast, phage, etc.), which can provide customized solutions for customers who need cell surface display.
An important idea for the development of human antibodies. Compared with other cells, the process of protein folding, secretion and post-translational modification in mammalian cells is most similar to that in the human body. The system can display the full-length human antibody, which has the potential to exert the effect function of antibody constant region. In addition to the regular plasmid, the display process can also be mediated by baculovirus.
Bacterial surface display system is an important branch of the microbial surface display system. It can be regarded as an alternative way of phage surface display to a certain extent. The basic strategy of bacterial surface display is to fuse or embed foreign proteins or peptides with bacterial surface proteins, and to combine active expression with the bacterial surface. Gram-negative bacteria autodisplay for E.coli and Gram-positive bacteria display for Lactococcus lactis and Bacillus subtilis are all available at Creative BioMart.
A protein engineering technology uses the expression of recombinant protein incorporated into yeast cell wall to isolate and engineer antibodies. Both Saccharomyces cerevisiae surface display service and yeast surface library service are available at Creative BioMart.
Phage libraries can be used for affinity screening of combinatorial peptide libraries to study protein-ligand interactions, characterize the binding sites of ligands, receptors and antibodies, define epitopes of monoclonal antibodies, select enzyme substrates and screen cloned antibody libraries. DNA encoding millions of variants of certain ligands (e.g., peptides, proteins, fragments) is cloned into phage genome in batches as part of one of phage coat proteins (PIII, PVI or pVIII). Large libraries containing millions of different ligands can be obtained by forced cloning in E. coli.
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