Yarawia lipobytica is a representative unconventional yeast. At present, the strain has been used to express 42 heterologous proteins. As an expert in protein expression, Creative BioMart has established a complete yeast expression platform. Based on this platform, we provide a simple method to clone and express the genes of interest in Yarawia lipobytica. Through the expression protocol we described, high levels of heterologous proteins can be expressed in cells or secreted from cells into the culture medium.
The expression protocol is simple:
We have developed two expression vectors for either cytosolic and non-glycosylated or normally glycosylated and/or secreted protein expression. Through Creative BioMart's Y. lipobytica system, the following missions can be achieved.
Strain advantage: compared with other yeasts, Y. lipobytica has a certain post-translational modification ability, higher secretion ability and product yield
Safety: the US Food and Drug Administration (FDA) lists Y. lipobytica as generally considered safe
Simple: the system is easy to operate, so the time and error are small
Stability: it has strong stability in vector and constructed plasmid
Flexibility: proteins can be expressed in cells or secreted from cells into the medium
High growth capacity: high secretory capacity and high product yield
Low protein degradation rate, suitable for large-scale production
Did not find what you need? In addition to the Yarrowia Lipolytica Protein Expression Service, we also offer
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
