Kluyveromyces lactis expression system has several advantages over other yeast and bacterial protein expression systems. The strain can rapidly reach high culture density and mass-produce recombinant proteins. For clients who intend to use K. lactic strain for short-term rapid secretory/non- secretory protein expression, we recommend Creative BioMart's Episomal Kluyveromyces Lactis Expression System.
We have developed the circular plasmids pKD1 as the expression vector for the episomal expression protocol.
pKD1 is derived from K. drosophilarium and transferred into Kluyveromyces lactis. It is extremely stable in host cells with copy numbers of 70-100 / cell. pKD1 is very similar to the 2μm circular plasmid of Saccharomyces cerevisiae, and has been commercialized as a vector.
To express the target protein, the GOI will be inserted into the MCS on pKD1. After replicating in the E.coli, the recombinant expression vector will be linearized with an appropriate restriction enzyme and then transformed into the K. lactis. A strong LAC4 promoter on the vector can be used to initiate gene expression. Using the inducible promoter LAC4, we can express secretory or non-secretory proteins for our customers.
In addition to the 2 μm stable high copy circular plasmid pKD1, we also offer expression services based on
Just let us know your requirement.
Our service is very flexible. Our main purpose is to make customers satisfied. Clients can start our expression project by submitting the target sequence online. By default, we use our original vector for cell line construction. Of course, you can also provide your vector and template sequence by mail or authorize us to purchase from other companies.
As the program is completed, clients will get (1) a vector sequencing report; (2) a protein expression test report; (3) a package that contains the recombinant expression vector, recombinant cell strain, and target protein of required concentration.
The protein expression service based on Episomal System is suitable for clients who want to use K. lactic strain for short-term rapid secretory/non-secretory protein expression. If it does not fit with your requirement, we also offer Integrated Kluyveromyces Lactis Expression System, which takes a longer time to construct but is competent for large-scale industrial fermentation.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
