Plant Virus Vector Delivery System

Tobamoviruses	The active TMV genomic RNA is produced by in vitro transcription of TMV cDNA to infect the host and produce systemic spread.
Potexviruses	The method of transformation is simple and high copy foreign genes can be obtained quickly. We mainly construct vectors by gene insertion and fusion protein strategy
Potyviruses	It is suitable for infecting Solanaceae plants
Bromoviruses	Cucumber mosaic virus (CMV) and alfalfa mosaic virus (AMV) are available as the vector
Comoviruses	CPMV expression cassettes and the sequence encoding P19 have been incorporated into the resulting vector, allowing high-level expression of multiple polypeptides by infiltration of a single construct
Geminiviruses	This viruses family has single-stranded circular DNA genomes that replicate to very high copy numbers in the nuclei of infected plant cells. It has the potential of using DNA containing geminiviruses as extra chromosome gene amplification vectors to express useful proteins in plants.

Why Select Plant Virus Vectors to Deliver the GOI

Compared with the Agrobacterium delivery method, the plant virus expression vector system has five advantages:

• The virus vector can replicate repeatedly in the host cells, which makes the foreign gene express at a high level.
• The virus proliferates rapidly, and the accumulation of foreign genes can reach the maximum in a very short time (usually within 1-2 weeks after inoculation).
• Most plant viruses can infect plants by mechanical inoculation, which is suitable for large-scale commercial operations.
• Plant viruses can infect monocotyledons and other non-host plants of Agrobacterium tumefaciens, expanding the scope of application of genetic engineering.
• Virus particles are easy to purify, which can significantly reduce the cost of downstream production.

Therefore, plant virus is an excellent choice for transient plant recombinant protein expression.

Related Services

In addition to the Plant Virus Vector Delivery method, we also provide other three delivery strategies for clients to choose from. Please click the links below if you are interested.

• The Biolistic Bombardment Nucleic Acids Delivery System
• The Agrobacterium Tumefaciens-Mediated Transient Expression System
• The Agrobacterium Rhizogenes-Mediated T-Dna Fragments Delivery System
• Production of VLPs in plants

With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.