After years of vigorous development, plant transgenic technology has been widely used in agricultural and plant biology research and the expression of a variety of medicinal proteins. Many biological products (such as bioactive peptides, therapeutic proteins) are produced from plant systems such as tobacco, tomato, rice and lettuce.
Lettuce is a diploid plant with autogenous ability. It has strong growth ability and is easy to grow, has a strong foundation in agriculture, and is an excellent host for medicinal protein production. Here, Creative BioMart has achieved robust plant-based protein production through Agrobacterium tumefaciens delivery, and has a wide range of applications. This method can rapidly produce a high level of recombinant proteins in a few days, and has the potential to expand to the agricultural level.
The transformation of a recombinant gene can be carried out in different tissues (including leaves and stems) of lettuce. For the transformation of leaf tissue after the infiltration of crops, the target protein can be expressed within 2 to 3 days. Then the transformed leaves are harvested and proteins extracted and purified. Compared with the conventional stable expression, the transient expression method can produce a higher protein expression level. In addition to higher expression efficiency, our internal factory also ensures that genetically modified products are not exposed to the environment, which is safe and environmentally friendly.
The plant cell we used is isolated from Lactuca sativa leafy cultivar by default, such as Black Seeded Simpson cultivar, Grand Rapids cultivar, or Oak Leaf cultivar.
We have a professional plant culture room, which can operate at 25 ℃. Climate control room has a humidity of 60 – 80% and pplants are under artificial fluorescent lamp for 16 / 8 hours.
The basic expression process includes the following steps:
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
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