Bacillus Subtilis Sugar Inducible Expression Service

Based on our bacterial expression technology platform, Creative BioMart has developed a sugar inducible Bacillus Subtilis expression service. For our clients, we can provide

• Expression of traditional antibody, heavy chain antibody, single chain antibody and in vivo antibody
• Vaccines against multiple pathogens of humans or other animals (for research only)
• Expression of natural or designed polypeptides with unique biological functions such as antibacterial, antiviral, antivenom and therapeutic effects
• Combined D-mannose induction and high cell density fermentation

We promise to provide clients with high-quality Bacillus subtilis sugar induced expression services.

Technology Description

Mannose is not only a specific and effective inducer of the two operons, but also transported by the manP gene of the manR operon, which is recognized as the component of PTS manR specific enzyme IIBCA and mannose transporter. Once the manP gene is deleted, the two operons will be constitutively activated, which lays the mechanical foundation of the protein.

Why Choose Our Service

• Extremely high success rate for almost hundreds of proteins and antibodies for the past decade of service.
• Multiple excellent strains and vector systems for regular and toxic products via intracellular accumulation or secretion expression.
• Optimal cultivation and induction at Creative BioMart with the whole range of scales from 5 mL to 1000 L per batch.
• One-stop service: from initial design and optimization all the way down to purification and further modification with well-recorded data and organized reports.

The original D-mannose-inducible expression of B. subtilis leads to a 4- to 7-fold increase of the EGFP expression with an optimal concentration of D-mannose. In addition to the manipulation of the carbon catabolite repression (CCR) and high-cell-density fermentation, the advanced expression system elevated the protein expression over 5 g/L subsequently. However, the incredible natural expression system possesses more potential beyond past comprehension. During years' trials and accumulations of experiences, scientists at Creative BioMart have released the hidden potential of B. subtilis greatly.

Related Service

Not the service you want? We also offer Auto-inducible Bacillus subtilis Expression Service. Please click the link if you are interested.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Creative BioMart's professional team will provide you with detailed guidance and solution ASAP.