An inducible expression system is a successful commercial system. It can be used to control the expression of protein or biochemical pathways in the cell factory. The two-stage production process of biomass accumulation and product accumulation can be realized from the promoter expression pathway induced at a specific time point. This method can improve the yield, titer and productivity, and improve the process economy. However, due to the limitations of some common inducible expression systems, not all target proteins are suitable for expression from the perspective of cost performance and feasibility. For example, your requirements may involve one or more of the following
Please consider Creative BioMart's auto-inducible expression system.. The tryptophan (Trp) expression system combined with T7 RNA polymerase is expected to meet your needs.
The operon of the tryptophan induction system is composed of 5 structural genes encoding tryptophan synthetase, which is regulated by TrpR. In the medium of excessive tryptophan, TrpR binds to trpO, blocking the transcription Ptrp from the operon. Inhibition is further enhanced by attenuation, a mechanism in which trpL forms RNA stem ring, preventing transcription in the presence of tryptophan.
In order to establish a low-level expression and low-cost induction system, we combine the high-level gene expression realized by T7 PCR with the strictly regulated Trp operon. With such a system, we hypothesize that the start of gene expression can be adjusted to a certain point in time by adjusting the amount of tryptophan added to the medium at the beginning of culture
The vector we designed contains tryptophan repressors and natural tryptophan precursor sequences, promoters and operators. The inhibition and induction of the self-induced expression system are controlled by tryptophan concentration. When tryptophan is present in the growth medium, it will be bound by TrpR, TrpR will bind to trpO and inhibit the expression of T7 polymerase and GOI. After tryptophan is consumed, the conformation of trpr changes, leading to dissociation from trpO. T7 PCR is produced and plasmid-based GOI is transcribed from pT7.
We offer a variety of flexible service packages for the choice of the client.
Client only needs to provide the target sequence and requirements. We will be responsible for the steps including codon evaluation, expression vector design, induced expression, expression condition optimization, protein product detection, and large-scale production.
We can also build an expression vector for you. Our library contains hundreds of compatible plasmid vector skeletons, which can build multiple vector candidates according to your needs.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
