Hairy roots induced by Ri plasmid of Agrobacterium rhizogenes carry all the genes of the plant. Agrobacterium rhizogenes has many characteristics to adapt to industrial production. Creative BioMart has developed a plant recombinant protein expression system based on Agrobacterium rhizogenes to produce secondary metabolites. The system does not need to add exogenous auxin, grows rapidly and autonomously, and has high genetic stability. Moreover, the content of exogenous protein is often higher than that synthesized by plants.
According to the actual expression requirements, we will complete the system construction in three ways:
Cut the wound on the sterile plant seedling and stem, inoculate the Agrobacterium Rhizopus in the wound, and after a period of time, hairy roots will be produced in the inoculation site.
The sterile explants of plants are cultured with Agrobacterium Rhizopus. The culture medium is selected for subculture. Agrobacterium rhizogenes are killed, the transformed cells produced callus and hairy roots are induced.
The callus is prepared into protoplast, and cultivated with Agrobacterium rhizogenes. The transformed cells are screened on the antibiotic-containing medium, and then transformed and cloned, and then the whole plant can be obtained using the differentiation medium.
In addition to the Agrobacterium Rhizogenes-Mediated method, we also provide other three delivery strategies for clients to choose from. Please click the links below if you are interested.
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
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