Grain Crop Transgenic Operation

Food crops are the main source of food for human survival. Based on the mature crop system, Creative BioMart provides standardized transgenic operation services for scientific research institutions and related high efficiency, and provides technical support for the field of genetics and breeding. Our transgenic plants mainly include rice, wheat, corn, potato and so on. Please note that the service is only for experimental research, not for actual production.

Rice (Oryza sativa L.) Transgenic Service 

Figure 1. Agrobacterium-mediated transformation of rice (Luu, 2020)

Service Content

Vector construction Induction and subculture of rice receptor callus Agrobacterium-mediated transformation and screening of rice Differentiation of positive callus into plantlets Rooting and strengthening of regenerated green plantlets PCR detection of a foreign gene in t0 generation transgenic seedlings

Provide Results

Images of genetic transformation and screening. The image of the callus differentiation and regeneration part of the sample. Agarose gel electrophoresis of PCR gene detected by T0 generation. Transgenic rice seedlings of T0 generation. Positive T0 generation seedlings of each transformation experiment.

Wheat (Triticum aestivum L.) Transgenic Service

The experiment will last about 6 months. The transgenic process is similar to that of rice strain and the provided results will include

• Progress description.
• Vector construction, sequencing report and pictures.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• The target gene PCR of T0 generation regenerated plants is detected by gel electrophoresis.

Corn (Zea mays L.) Transgenic Service

6-8 months required. The transgenic process is similar to that of rice strain and the provide results will include:

• Progress description.
• Vector construction, sequencing report and pictures.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• Target genes PCR of T0 generation are detected by gel electrophoresis or GUS.

Figure 2. Agrobacterium-mediated transformation (Luu, 2019)

Potato (Solanum tuberosum L.) Transgenic Service

4-6 months required. The transgenic process is similar to that of rice strain and the provide results will include:

• Progress description.
• Vector construction, sequencing report and pictures.
• Explant infection, callus induction, embryogenic callus, tissue differentiation, plant regeneration and so on.
• The target gene PCR of T0 generation regenerated plants is detected by gel electrophoresis.

For all the services above, customers can directly provide the constructed transformation vector, or entrust our company to construct various vectors such as gene cloning, expression and knockout. We will complete the whole experiment process from the construction vector to the acquisition of transgenic seedlings for you.

The Overview of the Biological Service Process

• Sign service contract;
• The client transfers the target gene and makes the payment;
• We carried out transgenic experiments;
• Deliver the experimental results to the customer, and the customer checks and accepts them.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

References

1. Luu, V. T., Stiebner, M., Maldonado, P. E., Valdés, S., Marín, D., Delgado, G., Laluz, V., Wu, L., Chavarriaga, P., Tohme, J., Slamet-Loedin, I. H. and Frommer, W. B. (2020). Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka. Bio-protocol 10(17): e3739.

2. Sunil Kumar B., Immanuel Selvaraj C. (2019) Agrobacterium-Mediated Transformation for Insect-Resistant Plants. In: Kumar Chakravarthy A., Selvanarayanan V. (eds) Experimental Techniques in Host-Plant Resistance. Springer, Singapore.