Tetracycline (Tet)-regulated gene expression is a eukaryotic expression system developed in recent years. It uses tetracycline and its derivatives to induce the expression of interested genes. This induced expression system has the advantages of strict, high efficiency, strong controllability and small expression leakage. It has been widely used in a variety of cell lines, plants, yeast, nematodes and small intestine mouse, rat and human cells. The Tet-inducible mammalian expression system is now available at Creative BioMart.
Tetracycline-regulated gene expression system was developed based on the tetracycline-resistant operon of E.coli Tn10. The basic principle is that inducing drugs such as Tet change the conformation of the regulatory protein, so as to control the expression of target protein, which belongs to negative regulation mode.
Various vectors containing tetracycline can be used to regulate gene expression systems. At Creative BioMart, we offer
It is a double plasmid controlled expression system, which is used in most eukaryotic cells
Lentiviral vector system is an efficient vector, which can introduce genes into mammalian cells permanently.
PiggyBac inducible gene expression vector system combines Creative BioMart's efficient piggyBac vector system and Tet on inducible gene expression system to help you realize the permanent integration of tetracycline inducible gene expression cassette based on transfection with host genome.
The expression process can be handled in biosafety level 1 facilities because AAV is inherently replication-deficient, producing little or no inflammation, and causing no known human disease.
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