AcMNPV was initially isolated from Autographa californica and then named after it. The basic principle of the AcMNPV expression system is to insert the coding gene of the target protein into the AcMNPV genome, and then use it to infect insect cells, directing the synthesis of the bioactive target protein.
As the best-studied insect cell expression system, various improvements have been conducted to AcMNPV expression system and Creative BioMart's insect cell expression platform is compatible with these comprehensive expression pathways. For customers who are interested in protein expression through insect cells, please contact us and we will recommend the most appropriate expression pathway for you.
AcMNPV-Based insect cell protein expression services offered by Creative BioMart include but are not limited to
Bac to Bac
Best-developed classic insect cell expression approach, the basic version of several other expression approach.
Mainly relies on Tn7 site-specific transposition between donor plasmid and Bacmid to generate recombinant baculovirus. E. coli cells are required as an intermediate host in this approach.
Application: basic protein expression
Bac to Bac TOPO
An approach improved from Bac to Bac. It shares the same advantage with Bac to Bac approach.
TOPO clone is applied to replace the conventional subclone step so that the foreign sequence can be inserted into the donor vector in 5 mins rather than 2-3 days.
Application: efficient recombinant protein expression.
Bac to Bac HBM TOPO
Another approach improved from Bac to Bac. Specially modified to produce secretory proteins.
In addition to TOPO clone elements that facilitate the rapid subclone, an N-terminal Honeybee Mellitin (HBM) secretion signal coding sequence is added to the donor vector to guide recombinant protein secretion into the extracellular medium.
Application: efficient secretory recombinant protein expression.
Baculovirus LR Recombination Expression Approach
An approach that utilizes LR recombination to intergrade foreign genes with an expression vector within 1 hour. Expression vectors infect the insect cells directly, eliminating the section of transforming bacteria, bacmid extraction, or co-transfection.
Application: production of high titer stock as well as highly efficient protein expression.
Rescue Expression Approach 2 (FlashBAC)
Nearly 100% recombinant rate guaranteed due to the rescue strategy. Inactivating baculovirus backbone by replacing essential elements and reactive the virus replication function by co-transfecting backbone and transfer vector.
Application: eliminate non-recombinant parental virus background.
Rescue Expression Approach 3 (BacPAK)
A faster baculovirus-based expression system, which eliminates the labor-intensive plaque screening and purification step. By partially replacing the essential ORF 1629 fragment with the bacterial artificial chromosome (BAC), the recombinant rate can be improved to nearly 100% thus avoiding screening and purification.
High-Throughput Virus-Free Approach
A plasmid-mediated expression approaches. The corresponding transfer vector is framed with a hr5 enhancer and an immediate early promoter ie1 to directly induce the transcription in host insect cells. Thus, the step from construction recombinant virus to virus amplifying can be eliminated. Target protein samples can be produced within 48 hours.
Application: high throughput protein expression, determining the optimal expression system for multiple target proteins and scale up by enlarging the culture volumes.
Besides the basic expression approaches mentioned above, Creative BioMart also expanded some featured services.
Multi Gene Expression
More than two expression domains can be inserted to express multiple proteins or complexes for the wide range of studies like protein interaction research.
Virus-Like Particles (VLPs) Expression Service
Creative BioMart offers conventional Bac to Bac method for the VLPs which contain 1 or 2 subunits and for multigene expression VLPs which contain more than 4 subunits.
N- Linked Glycoprotein Expression Service
A solution that can eliminate the baculovirus' limitation on N-linked glycosylation.
Kinase Expression Service
Protein kinases are mainly produced by baculovirus expression systems.
BacMam Service
Use baculovirus to mediate gene expression in mammalian cells.
Baculovirus Surface Display Service
Through infusing with certain virus transmembrane function protein, the target protein can be displayed on the surface of virus capsid, occlusion body, insect cells, and even mammalian cells.
If you have any questions or requests, please feel free to contact us or submit an online inquiry. Our customer service is always ready for you 24/7.
