Protein De Novo Sequencing Service

The limitation of the traditional protein sequence analysis technique is obvious — if the protein is not included in the database, then the protein cannot be sequenced. To address this problem, Creative BioMart provides a De novo protein sequencing service, through which researchers can check whether the pre-set post-translational modification has been performed correctly while sequencing the peptide chain.

Technology Description

Technology Workflow of Protein De Novo Sequencing Service

Different from traditional methods, de novo sequencing does not need to rely on any database to analyze the results. The peptide sequence can be directly deduced from the experimental MS/MS spectrum by some calculation methods. It can be used to assay the non-sequenced biological proteins, antibodies, post-translational modified (PTM) peptides and endogenous peptides.

Technology Workflow

Experimental design
DNA preparation
Sample quality test
Library Construction
Computer sequencing
Data quality control
Data analysis

Service Content and Deliverable

Creative BioMart's technology team provides you with one-stop genomic de novo sequencing technology services, including experimental design, sample preparation, on-line sequencing to bioinformatics analysis, aiming to provide solutions for your genomics. Our genome de novo sequencing services include:

Service content	Deliverable
De novo sequencing of animal and plant genomes	Experimental procedures (Chinese and English) Related mass spectrum parameters Mass spectrum images Original data Peptide sequence information Protein sequence and mutation information
De novo sequencing of bacterial genome
De novo sequencing of fungal genome
Metagenome de novo sequencing

Our Strengths

Platform advantage - obtaining high-quality genome map
High depth sequencing - high accuracy
Rich data analysis content - standard analysis, advanced analysis, customized analysis
Rich experience in genome sequencing and analysis - a number of large genome sequencing projects have been completed
Customized genome sequencing scheme - personalized genome sequencing scheme can be made according to the actual needs

Technology Applications

Sequence the peptides that do not exist in the protein library. It can be used to detect biological proteins and unknown proteins that are not sequenced, and to reverse engineer enzymes and other protein molecules.
It can be used to identify post-translational modifications and mutations, and accurately determine the protein sequence.
In combination with matrix-assisted laser desorption/ionization (MALDI) peptide fragmentation technology, protein identification can be performed on gel spot samples stained with gold or silver.
Combined with ESI peptide fragmentation technology, it can be used to identify complex samples such as adhesive strip samples (i.e. SDS-PAGE samples).

With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

Reference

1. Ma B, Johnson R. De novo sequencing and homology searching. Molecular & cellular proteomics, 2012, 11(2): O111. 014902.

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