Bacterial surface display system is an important branch of the microbial surface display system. It can be regarded as an alternative way of phage surface display to a certain extent. The basic strategy of the bacterial surface display is to fuse or embed foreign proteins or peptides with bacterial surface proteins, and to combine active expression with bacterial surface. In recent years, with the rapid development of technology, both Gram-negative (like E. coli ) bacteria and Gram-positive bacteria have been used as host cells for heterologous protein display. Currently, the technology has been widely used in microbiology, molecular biology, vaccinology and other fields.
Creative BioMart has rich experience in the field of bacterial display. We are able to offer a wide range of bacterial surface display service based on Gram-negative or positive strains. On top of that, we also provide several bacterial display scaffolds to meet the specific expression requirement.
Gram-negative bacteria surface display system is a well-developed cell surface display system, and E. coli is the most widely used strain. The passenger protein (target protein) can be expressed on the cell surface by fusing with the specific carrier protein (anchoring motif) of the host cell. We will select the carrier protein according to the size of the passenger protein and formulate the expression scheme. Here are some display scaffolds available at Creative BioMart.
| Scaffold | Type of fusion | Passenger size | Application | Verification of display |
| LamB | Insertional | 1.2 –25.5(kDa) | Bioremediation, peptide library screening, vaccine development | Cell fractionation, immunoblotting, functional assays |
| OmpA | Insertional | 1-50(kDa) | Peptide library screening, peptide display, vaccine development | Cell fractionation, immunoblotting, fluorescence microscopy, flow cytometry |
| Fimbriae (FimH and FimA) | Insertional | 1-4(kDa) | Peptide library screening, immunogenic peptide display, bioremediation | Functional assays, fluorescence microscopy |
| INP | C-terminal | 7-119(kDa) | Epitope mapping, biocatalysis, vaccines, protein library screening | Cell fractionation, immunoblotting, flow cytometry, functional assays |
| Lpp-OmpA | C-terminal | 27-74(kDa) | Bioremediation, biocatalysis, antibody library screening | Cell fractionation, immunoblotting, fluorescence microscopy, functional assays |
| AIDA-I (Autodisplay system) | N-terminal | 12-65(kDa) | Biocatalysis, protein library screening, vaccine development | Cell fractionation, immunoblotting, fluorescence microscopy, flow cytometry, functional assays |
In recent years, the research of using Gram-positive bacteria as surface display host has been rising. Compared with Gram-negative bacteria, Gram-positive bacteria grow faster and the transport of foreign protein only needs to pass through one layer of the membrane. Our Gram-positive bacteria surface display services include but are not limited to:
The applications of bacterial display include but are not limited to:
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.
Reference
Kotrba P., Rulíšek L., Ruml T. (2011) Bacterial Surface Display of Metal-Binding Sites. In: Kotrba P., Mackova M., Macek T. (eds) Microbial Biosorption of Metals. Springer, Dordrecht.
