Rabbit Reticulocyte Lysate Protein Synthesis Service

Reticular cells are highly specialized cells which mainly responsible for the synthesis of hemoglobin. Hemoglobin accounts for more than 90% of the proteins in reticular cells. These immature erythrocytes have lost their nucleus while the mRNA and complete translation function still remained to carry out extensive globulin synthesis.

Rabbit reticulocyte lysate (RRL) is an efficient eukaryotic protein synthesis system in vitro. It is an alternative system of the Wheat Grem Extract Cell-free Protein Synthesis System. RRL can realize post-translational processing such as isoprene, acetylation, N-myristoylation, phosphorylation, ubiquitin binding, signal peptide processing and core glycosylation.

Click Here To See The comparison of each cell-free protein synthesis systems→

The system is widely used in the identification of large mRNA types, analysis of gene product properties, transcription and translation regulation, and co-translation processing.

Features of the RRL system

Compared with the traditional cell/cell-free protein synthesis platform, the RRL system has many advantages, including:

• Easy preparation
• No detergent needed
• Translation independent of the upper limit
• Compatible with mammalian post-translational protein modification system
• It can express large proteins of more than 30 kDa effectively
• Compatible with high-throughput formats for protein microarray and other screening programs

Creative BioMart has developed translation systems (mRNA-based) and coupled transcription/translation systems (DNA-based) that are based on rabbit reticulocyte lysate. Through continuous cell-free exchange expression, the yield of recombinant protein may reach gram level. Please click the links below to find out more information.

Rabbit Reticulocyte Lysate System for mRNA

Service is suitable for protein expression of either in vitro transcribed mRNA or mRNA isolated from tissues or cells. The system can be used to

• Express single proteins as well as multiple proteins in high-throughput applications such as display technologies.
• Analyze RNA functionally and structurally
• Study aspects of the translational machinery.

Rabbit Reticulocyte Lysate System for DNA

Linear and plasmid DNA is the template in the system. The process of template transcription and translation is coupled together to construct an efficient system. T7, T3 or SP6 RNA polymerases can all be used to regulate the reaction.

The choice of the system is dependent on several factors, including the origin of the template RNA and DNA, protein yield or whether the protein of interest requires post-translational modification. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.