Drosophila Genetic Operation Service

The binary expression system is a simple and efficient genetic operation tool, which improves the recombination efficiency of specific genes and realizes the precise expression of genes in time and space. Drosophila melanogaster has become a favorite in the field of scientific research because of its application of binary expression system. In the experimental study, researchers can mark tissue or cell by precise genetic operation, improve the specificity of the experiment, and achieve the goal of precisely regulating a gene in tissue or cell.

Figure 1. GAL4/UAS system for modular expression (Halesl, 2015)

Creative BioMart can provide Drosophila Genetic Operation Service based on the binary expression system of Drosophila melanogaster. For clients who are interested in our services, please see below for more information.

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At present, binary expression system is one of the important tools to realize precise genetic manipulation. Binary expression systems are usually composed of two components that regulate gene expression:

(1)Tissue-specific activators of transcription

(2) Target genes controlled by upstream activation sequence

When the tissue-specific activator of transcription binds to the upstream activation sequence, the target gene is expressed.

Currently, the available binary expression systems at Creative BioMart include GAL4/UAS, FLP/FRT, LexA/lexAop、Q system, Cre/ loxP, and CRISPR/Cas9 system.

FLPFRT system for modular expression Figure 2. FLP/FRT system for modular expression (Halesl, 2015)

Comparison of Each System

For clients who have difficulties to decide his/her suitable expression method, please browse the information below. Hope these tips can help with making the decision.

Systems	Advantages	Limitations
GAL4/UAS	GAL4and U4S strains are abundant for researchers to choose from. GAL4 expression can be simply regulated by GAL80 or heat shock.	At present, it is used to label a single cell or a small group of cells Cell-specific GAL4 strains are limited
FLP/FRT	Gene deletion, translocation or inversion can be realized by site-specific manipulation	The spatial and temporal specificity is poor Regulating gene expression by heat shock and other physical and chemical methods may cause lethal effects.
LexA/lexAop	It is independent of GAL4 / UAS system and not affected by GAL80	The efficiency is lower than GAL4 / UAS and Q system In the absence of LexA, the transcription level of lexAop is high.
Q system	It is independent of GAL4 / UAS system and does not affect each other; Label and manipulate a single cell or a small group of cells In the absence of QF, the transcription level of QUAS is low.	The specific QF strains are less Drosophila; The construction of Drosophila needs to introduce 8 genes, and the operation is complex
Cre/ loxP	Cre recombinase is more resistant to high temperature than FLP recombinase.	The optimal temperature of Cre recombinase is significantly higher than the standard living temperature of Drosophila.

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

Reference

1. Hales KG, Korey CA, Larracuente AM, Roberts DM. Genetics on the Fly: A Primer on the Drosophila Model System. Genetics. 2015 Nov;201(3):815-42.

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