Hansenula Polymorpha Protein Expression Service

The protein expression service based on Hansenula Polymorpha is now available at Creative BioMart. The Hansenula Polymorpha yeast is a methyl nutritious yeast, which can be used as the only carbon source. In the H.Polymorpha expression system, the AOX and DHAS promoters are very strong. This characteristic makes Hansenula polymorpha an excellent host for the production of recombinant proteins for industrial applications and research purposes.

Why Choose Hansenula Polymorpha System

Figure1. Main advantages of Hansenula polymorpha (Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production, 2019)

• Highly heat resistant yeast (up to 50 ° C or higher)
• Stable high replica integration, up to 120 replicas per cell
• Lack of allergens, toxins and virus contamination
• Large toolbox, including inducible strong promoters
• Stable co-expression of multiple genes
• Provide high yield and simple purification method
• Metabolic engineering of the whole pathway is possible

Service Contents

Generation and amplification of expression vector	Timeline
Protein gene sequence analysis. Codon optimization.	1 week
Linearization vector and electroporation into host cell
The recombinant plasmid is linearized after sequence verification. The positive clones are screened by electroporation. At least 6 clones are selected for expression test.	1 week
Optimization of expression conditions
Expression conditions test: include temperature, medium, inducer type, induction time and induction amount. SDS-PAGE or Western blot is used to analyze the expression effect by default.	1 week
Amplification, expression and purification of target protein
Reaction system amplification Purity detection by SDS-PAGE Correctness detection by Western blot.	1-2 weeks

Available Promoters

P_AOX: very strong promoter that is repressed on glucose and induced during growth on methanol

P_TEF: constitutive promoter, same expression on glucose and methanol

P_AMO : promoter induced by primary amines (methylamine, ethylamine) and fully repressed by ammonium sulfate

P_ADH1: constitutive promoter, comparable to TEF on glucose but higher on methanol

Related Services

Did not find what you need? In addition to the Hansenula Polymorpha Protein Expression Service, we also offer

• Hansenula Polymorpha Surface Display Service
• Pichia Pastoris Protein Expression Service
• Saccharomyces Cerevisiae Protein Expression Service
• Kluyveromyces Lactis Protein Expression Service
• Yeast Surface Display Services
• Yarrowia Lipolytica Protein Expression Service

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.