Plant chloroplast genetic transformation and expression system is a new recombinant protein expression system developed in recent years. Compared with traditional nuclear transformation, chloroplast genetic engineering has many advantages, such as high expression of foreign proteins, simultaneous expression of multiple genes, no gene silencing phenomenon and good environmental safety. Creative BioMart is now able to conduct the protein production service in the chloroplast system.
Compared with the plant nuclear expression platform, tthe chloroplast expression system has several advantages:
Based on the conventional expression protocol, we use the chloroplast specific promoter, terminator, 5 '- UTR and 3' - UTR as the regulatory sequences of foreign genes to construct the expression vector together with the foreign genes, the expression cassette of selective marker genes and the homologous recombination fragments from chloroplast. After the expression vector is introduced into chloroplast by particle bombardment or PEG method, the target gene fragment is inserted into the chloroplast genome by homologous recombination and site-specific integration. Finally, homogenous plants are obtained by selection pressure and multiple rounds of screening to achieve the integration and high-efficiency expression of foreign genes.
The transformation process is mainly divided into four steps
We usually use the promoter of psbA gene and Prrn of 16S rRNA operon.
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
Reference
