Autodisplay is a genetic engineering technique that is developed based on the natural secretion mechanism of the AIDA-I autotransporter protein. By fusing with AIDA-I, the protein of interest can be displayed on the outer surface of the Gram-negative bacteria.
Autodisplay system is capable of 12-65 kDa protein display. For clients who want to display moderate to large size protein, please consider Creative BioMart's Gram-negative bacteria autodisplay service. The autodisplay service can be subjected to high-throughput screening (HTS) methods such as ELISA or FACS, enabling the screening of surface display libraries and providing access to directed evolution of the recombinant protein displayed at the cell surface.
The autotransporter pathway of Gram-negative bacteria is very outstanding and simple. The system consists of (1) an N-terminal signal peptide typical for the Sec pathway; (2) an autotransporter formulated with β-barrel of AIDA-I and a linker region. The passenger protein (protein of interest) will be fused to the region between (1) and (2).
By using a typical signal peptide, a precursor protein is transported across the inner membrane. Arriving at the periplasm, the C-terminal part of the precursor folds as a porin-like structure, as a so-called β-barrel within the outer membrane and the passenger is transmitted to the cell surface.
If not specifically required, we tend to use E. coli as the host. Compared with other surface display systems described to date, there are some striking advantages.
The autodisplay technology allows numerous applications in the area of biocatalysis, bioanalytics, screening and separation. The applications of autodisplay include but are not limited to:
