Protein Purification Service

Creative BioMart provides protein separation and purification services based on a variety of protein expression systems. Over the years, we have been committed to the research of protein expression and purification. We have comprehensive protein purification technology and a variety of purification systems, so that can provide a variety of protein purification schemes. Our service scope can be divided into natural protein purification service and recombinant protein purification service. Our service advantages include

The technology platform has first-class equipment to support diversified purification methods.
The service is suitable for recombinant proteins or natural proteins from different sources (mammals, plants, insects, bacteria, yeast, algae, acellular systems, etc.)
Professional technical team customizes the best purification scheme for the client
The purification process is transparent, and a comprehensive analysis report can be provided after the completion of the project

Natural Protein Purification Service

Project objective	Purification scheme
For natural small molecular protein	The strategy of multi-stage separation and filtration combined with molecular sieve is used to remove macromolecular impurities. The small molecular protein is purified by combining macroporous resin with reverse resin. The results of purification will be identified by HPLC and MS.
For natural macromolecular protein	Macromolecules with appropriate pore size are selected for separation and purification. Anion and cation adsorption columns are used with HLPC to identify and collect the corresponding elution peaks.
For mixed unknown protein samples	Make plans according to the specific situation of the project. The products are identified by mass spectrometry and activity identification.

Recombinant Protein Purification Service

Project objective	Purification scheme
For recombinant protein	Purification method----commercial affinity purification resin, such as Ni column, GST beads, etc Purification target----with 6xHis, GST, SUMO, MBP fusion protein Deliverable---- the purity of fusion protein is more than 90%
For recombinant protein without label	Antibody affinity purification. The purification method similar to natural protein can also be selected, depending on the project
Denaturation, renaturation and purification of inclusion bodies	Purification methods: urea, guanidine hydrochloride, detergent, extreme pH, etc. According to the properties of the protein, the denatured protein in the monomer state can be obtained to the greatest extent.

Project Workflow

Please refer to the following table for our service process. Please note that the actual experimental process may be slightly adjusted according to the specific situation.

Service step	Service content	Timeline
Protein detection and pre-experiment	SDS-PAGE is used to detect the protein; Small volume purification and detection	1-2 weeks
Protein purification and quality test	Large scale purification SDS-PAGE WB test Concentration test	1-2 weeks
Deliverable	Purification report Picture and data Purified protein

With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.

CONTACT OUR TEAM