Lentivirus belongs to retrovirus. Its genome is linear double positive strand RNA. Lentiviral vectors can efficiently mediate stable integration of foreign genes into mammalian cells. Due to the flexibility of foreign gene and promoter selection and the wide range of transfected cell types (such as primary cells, stem cells and non-dividing cells), lentivirus system has become one of the most popular methods to develop stable expression cell lines.
See the comparison of plasmid and viral vector systems→
Creative BioMart provides lentivirus-mediated stable cell line establish service for our customers. The service content includes virus packaging, virus particles purification, stable cell line establishing and delivery service and protein expression.
The recombinant lentiviral vector containing foreign sequence is transfected into packaging cells together with auxiliary plasmid. The packaged live recombinant lentivirus can be collected in the supernatant. After purification, the virus is used to infect cell lines that are difficult to be transfected by traditional transfection agents, such as primary cells, suspension cells and non-dividing cells. After infection, the foreign gene can be integrated into the genome of host cells for long-term stable expression.
Service Content
Plasmids preparation (<1 week)
Co-transfection (Day 1)
Co-transfection of transfer plasmid and auxiliary plasmid into packaging cells
Collection (Day 3-4)
Collect live virus from supernatant
The collected viral particle will be purified through Creative BioMart’s advanced lentiviral particle purification technology to obtain the high purity recombinant lentivirus for stable cell line establishment.
The purification process of lentivirus is as follows:
Ultracentrifugation: remove serum protein, host protein, plasmid DNA and host DNA
Ultrafiltration: further remove plasmid DNA and host DNA, further concentrate virus
Need high purity product? Our technology also supports ultrapure and extremely purity virus particles production. If you have any requirements, please contact us.
| Transfection | 4 weeks | Deliverable
Inquiry |
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| Stable Cell Line Construction |
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2 months |
Compared with other virus-mediated stable expression systems, the lentiviral system has the features of
lentivirus can achieve long-term and stable expression of the target gene by integrating foreign gene into host cell genome. It is the first choice of cell experiment;
No pathogenicity was found, and it has been used in CAR-T treatment;
Direct injection of living tissue is not easy to cause immune response, making lentivirus system also suitable for animal experiments.
Purity, titer and the sequence accuracy of recombinant virus particles are guaranteed.
Viral key protein removed, the virus cannot be assembled after transfected and integrated into the cells.
Stable integration into the cell genome, can be used to prepare a variety of the stable cell lines.
Creative Biomart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.
