Bacillus subtilis spore surface display (BSSD) is considered to be one of the most promising methods to express heterologous proteins with high activity and stability. Bacillus subtilis can form spores under adverse conditions. The spore shows resistance to ultraviolet rays, toxic chemicals, decomposing enzymes and extreme temperatures, making the bacteria extremely stable.
Creative BioMart has established a complete BBSD procedure.
The intended exogenous protein will be located on the surfaces of spores through the fusion vector of anchor protein and target protein. The recombinant vector containing heterologous protein and anchor proteins will be transformed into host strain B. subtilis. After inducing sporulation, the target protein can be displayed on the surface of spores. The displayed proteins have the ability to be active in extreme environment.
The foreign protein can be fused with the N-terminal, C-terminal and sandwich structure of the anchor protein. We usually use CotB, CotC and CotG as anchor proteins to fuse with foreign proteins. Their applications are as follows:
| Anchor Proteins | Features and Application |
| CotB |
|
| CotC |
|
| CotG |
|
*If required, we can also fuse other anchor proteins for you, such as CotB, CotC, CotG, CotZ, CotX CotY, CotA, OxdD, CotE, CotZ, CgeA and other capsid proteins. Just let us know in advance.
At Creative BioMart, BSSD can be performed by recombination and non-recombination fusion.
Non Recombination Fusion Solution
BSSD can be performed in a non-recombination way. We culture the spores with purified foreign proteins together and immobilize the protein on the spore surface by electrostatic interaction, hydrophobic interaction or cross-linking agent. Usually, two types of vectors are used in the construction of recombinant proteins, such as episomal vectors and integrating vectors.
Recombination Fusion Solutions
Creative BioMart's recombinant fusion solutions are based on the integration vector. We offer both single and double crossover recombinant solutions for our clients.
The homologous arm needed for single crossover is only one-ended and short, but its integration efficiency is high and stable.
In the double exchange method, homologous recombination is achieved through homologous sequences located on both sides of the foreign gene and the target sequence of the host genome.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Creative BioMart's professional team will provide you with detailed guidance and solution ASAP.
