Bacillus Subtilis Expression System

Bacillus subtilis is a widely studied Gram-positive bacterium. The system can express secreted protein without endotoxin. Up to now, nearly half of commercial protease and amylase are produced by Bacillus subtilis.

The Bacillus Subtilis expression system is now available at Creative BioMart. We highly recommend that customers choose our services. In addition to the basic protein secretion expression service via SURE and SPCA systems, we also offer protein tagging service, food-grade protein expression service, Bacillus Subtilis display service, etc. For more detail, please find the information below.

Technology Features

Bacillus Subtilis is considered to have great potential as a host for the production and secretion of recombinant proteins.

Cell endotoxin-free, no pyrogenic reaction in mammalian.
Bacillus Subtilis is non-pathogenic, so it has been granted the Generally Recognized As Safe (GRAS) status by the US Food and Drug Administration.
The downstream protein processing is much easier than that of E.coli system due to its large secretory capacity.
Well-developed fermentation and product recovery technologies corresponding to the Bacillus Subtilis system.
Low codon preference.
High yield and good biological activity.

What We Can Do For You?

Several Bacillus Subtilis-related services are now available at Creative BioMart. These services include but are not limited to:

Creative BioMart's expression platform has a variety of Bacillus subtilis strains and corresponding expression vectors suitable for secretion expression, which can meet the different expression needs of customers. We use an efficient and rapid transformation method to ensure the positive rate of transformation and greatly shorten the transformation time.

General Service Content

Bacillus Subtilis Expression System

Service Name	Service Description	Timeline
Expression system selection	Expression form selection: intracellular expression/secretory expression Bacillus subtilis strains and vector selection	——
Preparation of target DNA	Codon optimization Gene synthesis	< 1week
Subclone construction	Subclone the target sequence to the vector Screening positive clones Clone sequencing to determine ORF	1-3 weeks
Protein expression and purification test	Transformation of the host strain Expression condition test Qualitative and quantitative analysis of protein products	2 weeks
1-10L expression and purification	Affinity purification Qualitative and quantitative analysis of protein products	1-2 weeks
Large-scale fermentation and purification	Protein affinity purification Protein buffer replacement and concentration Identification of purified protein using SDS-PAGE/WB	2-12 weeks depends on the required quantity
Deliverable	Cloning vector QC data Final report Protein product (quantity as per customer requirements)

Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP. If you have any special needs or are interested in learning more about Creative BioMart's protein expression services, please feel free to inquire about more details.

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