Stable Mammalian Expression System

Technology Description

Stable cell lines can be obtained by stable transfection. The process of stable transfection is to integrate the foreign gene into the genome of the cell so that the foreign gene becomes a part of the cell gene and can be replicated.

The basic process of cell line development including gene synthesis and vector construction, transfection and evaluation, cell pool screening, pressure screening, high expression monoclonal antibody screening, stability testing, delivery of cell lines. To get stable cell lines with high expression, we need to pressure screen the cell pool and our platform supports methods like GS and DHFR.

Service Outline

Examples of Customers' Requirements and Our Solution

• Foreign genes need to be integrated into cell chromosomes.

Solution: Gene knockout, gene insertion mutation screening and other methods to modify the genome.

• Eliminate individual differences between cells to prevent them from interfering with the experimental results

Solution: Screening of monoclonal genetic strains.

• The foreign gene was not integrated into cell DNA, resulting in the disappearance of exogenous gene fragments after injection into the animal

Solution: It is necessary to inject cells expressing foreign genes into animals

• Some proteins are very stable while the transient RNA interference cycle is short, so the expressed target proteins cannot be extracted.

Solution: The gene interference effect of power consumption can be realized by screening stable genetic strains.

Our Strength

• Efficient screening process —— We provide several production optimization solutions like DHFR and GS selection systems to facilities the establishment of an efficient stable expression system.
• Strict cell source —— The cell source can be traced, which is helpful to correct mistakes in time.
• Support multi-product expression —— Cell line can be used to express recombinant monoclonal antibody, protein, antibody or protein fragment.
• Stable and high yield.
• Short expression period —— It only takes 3-4 months from DNA sequence provided by customers to protein products.
• One-stop service.

Cell Transfection Service

Transfection is the most essential step of stable cell line development. Creative Biomart offers comprehensive transfection methods that match customer requirements.

See Features and Comparison of Virus and Plasmid-Mediated Mammalian Expression Systems→

Creative Biomart is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have any questions, please feel free to contact us or submit an online inquiry.