Surface display antibody library technology is one of the most popular technologies for the preparation of human antibodies. Mammalian cell surface display system has become an important direction for the development of human antibodies. Compared with other cells, the process of protein folding, secretion and post-translational modification in mammalian cells is most similar to that in the human body. Mammalian cell surface display system can display the full-length human antibody, which has the potential to exert the effect of antibody constant region.
Mammalian cell surface display has exhibited the following features over other systems:
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Multiple heavy chain and light chain primers can be designed to achieve rapid amplification through PCR reactions. The full-length antibody library with a capacity of 1010 could be constructed within 2 weeks.
Baculovirus is used to express the target protein in mammalian cells as vector. The interaction between virus GP64 gene and mammalian cell membrane is used to display the surface of baculovirus.
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