For the whole process of recombinant protein expression, the cloning of the target gene and construction of the vector is a routine and key procedure. The successful construction of the vector is the prerequisite for the subsequent protein expression. With the development of genetic engineering, more and more expression elements have been developed for recombinant protein production and plant genetic transformation. For different traits of crops, it is necessary to combine the expression elements on the vector to achieve the best effect. In addition, the correct selection of expression constructs also plays an important role in the analysis of gene function and promoter activity.
Creative BioMart is an expert in plant genetic engineering and molecular biology. Over the years, we have been committed to the development and optimization of plant expression vvectors, and constantly improving the construction pprocess of the expression vector.
In addition to one-stop plant expression system construction and recombinant protein expression services, we also provide customized plant expression vector construction and optimization services. Our services include target sequence design, sequence synthesis, DNA fragment assembly, sequencing, plasmid preparation, etc.
Here are some available vector construction services for customers to select from：
| Construction Purposes | Optional expression elements and vectors |
| Overexpression vector construction | We offer fully customized vectors that harbor specific promoters, including:
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| Silencing vector construction | We offer
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| Gene knockout vector construction | The cas9 gene sequence has been ccodon-optimized to improve the expression in plants. AtU6 promoter drives sgRNA expression in dicots and OsU3/OsU6 drives sgRNA in monocots. |
| Promoter activity analysis vector construction | Your target promoter will be fused with the GusPlus gene for histochemical GUS staining use |
| Complementary expression vector construction | Native promoter drives gene expression. |
Before the project starts, we will also provide consulting services for you. With advanced technology and experienced staff, we will work closely with you to provide satisfactory plant expression vector construction services.
With our professional research and development team and the latest protein expression and purification resources, we promise to deliver high-quality products for our customers. Customers who need our assistance are more than welcomed to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
