Synechococcus Elongatus is a versatile model cyanobacterium for study or biological production. S. elongatus PPC 7942 was the first cyanobacterium demonstrated to be reliably transformable by exogenously added DNA.
For customers who are looking for an excellent system for studying photosynthesis, circadian rhythms, nutrient regulation, environmental response, and lipid metabolism, please consider Creative BioMart's Synechococcus Elongatus Expression System.
S.elongatus has small genomes, thus easy to be genetically manipulated by natural transformation. To improve cell productivity and purifiability, Creative BioMart has developed an enhanced expression vector for the constitutive expression in the system.
We use psbA1 to stably and efficiently start the synthesis of foreign protein. The vector has flexible polyclonal sites. In order to facilitate the detection and purification of the protein, the N-terminal and C-terminal of the vector are labeled respectively. If you are interested in this technology, please contact us for the vector map.
| Service Name | Service Description |
| Expression strategy design | Client provides the target sequence and expression strategy designed referring to customers' requirements and protein properties |
| Codon optimization and gene synthesis (1 week) | Optimize the codon in order to improve protein yield significantly Use PCR to amplify the target fragment and link it to the expression vector |
| Construction of the expression cell strain (2 weeks) |
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| Protein expression, evaluation and optimization (1 week) |
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| Scale-up and protein purification (2-3 weeks) | We will scale-up your protein expression using optimized conditions. After multistep protein purification, the protein purity will be >90% |
| Deliverable |
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Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
