Phaeodactylum tricornutum belongs to the Bacillariophyta. P.tricornutum has obvious advantages as a bioreactor. It does not contain endotoxin, easily to be cultured and has a low cost. Creative BioMart has developed a protein expression protocol based on Phaeodactylum tricornutum, which can achieve high-level chloroplast expression of foreign genes through a site-specific transformation using chloroplast homologous recombination sequence. Creative BioMart is committed to providing our clients with one-stop service from gene cloning to protein expression and purification in P. tricornutum system. With our professional technical personnel and R & D team, high-value protein production will be guaranteed.
In order to achieve efficient expression, we constructed an expression vector. The vector contains two homologous sequences of chloroplast of Phaeodactylum tricornutum, which can be inserted by homologous recombination. The expression elements between homologous arms include chloramphenicol resistance gene for screening, CaMV 35S promoter, target gene fragment and downstream 250 BP NOS terminator. We will integrate the foreign gene expression cassette into the chloroplast genome by homologous recombination using Phaeodactylum tricornutum as a receptor. Electroporation technology is used to transfer the vector into the chloroplast of the recipient cells, and the transformed algae are screened out by chloramphenicol resistance. After expanded culture, the expression products can be detected. In this study, electroporation is used to transfer foreign genes into the chloroplast of algae cells, which can greatly reduce the operation cost and is easy to operate.
| Service Name | Service Description |
| Expression strategy design | Client provides the target sequence and expression strategy designed referring to customers’ requirements and protein properties |
| Codon optimization and gene synthesis |
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| Construction of the expression cell strain |
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| Protein expression, evaluation and optimization | Large number of transformants with high expression of recombinant protein are cultured in liquid medium |
| Deliverable |
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