Model Crop Transgenic Operation
The selection and utilization of model organisms are of great significance for genetic analysis, gene cloning and functional research. Creative BioMart has developed gene manipulation services for two common model organisms, tobacco and Arabidopsis, for scientific research institutions. We can achieve rapid genetic transformation by Agrobacterium mediated method. Customers can provide plasmids, Agrobacterium and so on. We can complete the whole experimental process from constructing vectors to obtaining transgenic seedlings for you. Please note that the service is only for experimental research, not for actual production.
Tobacco (Nicotiana tabacum L.) Transgenic Service
The experimental period is 3-4 months.
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Service Content |
- Vector construction
- Acquisition of tobacco aseptic seedlings
- Agrobacterium-mediated transformation and screening of tobacco
- Callus induction and adventitious bud differentiation of positive leaf disc
- Rooting and strengthening of regenerated green plantlets
- PCR detection of a foreign gene in t0 generation transgenic seedlings
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| Service Description |
Transgenic experiment
- Receptor material varieties: we provide our own varieties and other varieties as receptor materials.
- Type of receptor material: tobacco leaf.
Number of experiments
- Vector construction: one vector is constructed for each foreign gene.
- Gene Transformation: each transgenic vector is transformed into one receptor material, once.
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| Provide Results |
- Images of genetic transformation and screening.
- Differentiation and regeneration of adventitious buds.
- Agarose gel electrophoresis of PCR gene detected by T0 generation.
- Five or more lines are obtained in each transformation experiment.
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Agrobacterium tumefaciens-mediated transformation of tobacco can transform benthamia tabacum and Nicotiana tabacum. The fastest cycle is 3 months. Customers can provide plasmids, Agrobacterium and so on. We can complete the whole experimental process from constructing vectors to obtaining transgenic seedlings for you.
Arabidopsis thaliana Transgenic Service
Agrobacterium tumefaciens-mediated transformation has been used for Arabidopsis genetic transformation. The expression cycle is about 2-3 months.
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Service Content |
- Vector construction
- Plant culture of Arabidopsis thaliana
- Agrobacterium mediated transformation and screening
- Selection of T0 generation seeds
- Pure line screening and PCR detection of foreign gene
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| Service Description |
- Receptor materials: Columbia and other varieties are receptor materials.
- Number of experiments: each transgenic vector is transformed into one receptor material once.
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| Provide Results |
- Images of genetic transformation and screening.
- Agarose gel electrophoresis of PCR gene in T0 transgenic seedlings.
- T1 generation transgenic seeds: T1 generation seeds of 5 or more lines are obtained in each transformation experiment
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The Overview of the Biological Service Process
- Sign service contract;
- The client transfers the target gene and makes the payment;
- We carried out transgenic experiments;
- Deliver the experimental results to the customer, and the customer checks and accepts them.
Customers who need our assistance are more than welcome to contact us or submit an online inquiry. Our professional team will provide you with detailed guidance and solution ASAP.
