Product Name
CHO (Chinese hamster ovary) Cell
Expression System
Mammalian Expression System
Application
As mammalian host for the production of recombinant protein therapeutics.
Species
Female Cricetulus griseus (hamster, Chinese)
Media Adaptation
DMEM: Ham’s F12 medium (mixture 1:1) supplemented with L-glutamine and 5% fetal bovine serum
Split Ratio
1 × 104cells/cm2 will yield in a confluent layer in about 4 days
Seeding density
A ratio of 1:4 to 1:8 is recommended
Fluid Renewal
2 to 3 times weekly
Freezing Recovery
After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hrs
Sterility
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative
Growth Properties
Monolayer, adherent
Shipping Conditions
Dry Ice
Description
CHO cells are used in studies of genetics, toxicity screening,nutrition and gene expression, particularly to express recombinant proteins.
Industry Terms
Not Available to Industry
