Product Name
CHO (Chinese hamster ovary)-k1 Cell
Expression System
Mammalian Expression System
Application
As mammalian host for the production of recombinant protein therapeutics.
Species
Female Cricetulus griseus (hamster, Chinese)
Split Ratio
1 × 104 cells/cm2 will yield in a confluent layer in about 6 days
Seeding density
A ratio of 1:4 to 1:8 is recommended
Fluid Renewal
Every 2-3 days
Freezing Recovery
Allow the cells to recover from the freezing process for at least 24 h.
Sterility
Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative
Growth Properties
Monolayer, adherent
Shipping Conditions
Dry Ice
Description
The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957. The cells require proline in the medium for growth.
Industry Terms
Not Available to Industry
