Non-T7 Expression, Protein Expression in E. Coli, Protein Expression
Cell Strain
BL21
Species
E. coli
Genotype
fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10
Initial Medium
SOC Outgrowth Medium
Competent Cell Type
Chemically competent
T1 Phage - Resistant (tonA)
Yes,Resistant to fhuA2
Transformation Efficiency
0.6-1 × 109 cfu/μg
Transformation Efficiency Level
High Efficiency (> 109 cfu⁄µg)
Improves Plasmid Quality
Yes
Storage
SOC Outgrowth Medium(4°C) pUC19 Vector(-20°C) Competent E. coli (-80°C)
Shipping Conditions
Dry Ice
Feature
Versatile non-T7 expression strain,protease deficient.Chemically competent E. coli cells suitable for high efficiency transformation and protein expression.
Description
1.SOC Outgrowth Medium,1 X 25ml 2.pUC19 Vector (50 pg/µl), 1 X 0.025ml 3.Competent E. coli , 6 X 25ml
Industry Terms
Not Available to Industry
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