Product Name
DH10B T1 Phage-Resistantv Cells
Expression System
Bacterial Expression System
Application
Bacteria Competent Cells
Screening Method
Blue/White Screening
Cloning Methylated DNA
Yes
Competent Cell Type
Chemically competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
yes
T1 Phage - Resistant (tonA)
yes
Transformation Efficiency
> 1 × 109
Transformation Efficiency Level
High Efficiency (> 109 cfu⁄µg)
Improves Plasmid Quality
yes
Feature
1. tonA genotype to confer resistance to T1 and T5 phage
2. Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries (1,2)
3. The endA1 mutation to increase plasmid yield and quantity
4. ZΔM15 for blue/white screening of recombinant clones
Description
Store in ultra-cold freezer (-68 to -85°C).
Industry Terms
Not Available to Industry
