Product Name
DH5α Competent Cells
Expression System
Multiple Expression System
Application
Bacteria Competent Cells
Screening Method
Blue/White Screening
Cloning Methylated DNA
No
Competent Cell Type
Chemically competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
yes
T1 Phage - Resistant (tonA)
no
Transformation Efficiency
> 1 × 108
Transformation Efficiency Level
Medium Efficiency (108-109 cfu⁄µg)
Improves Plasmid Quality
yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Shipping Conditions
Dry Ice
Feature
1. Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
2. Maintains plasmid DNA with minimum recombination events
3. Delivers high-yield plasmid preparations for downstream applications
4. M13mp cloning vectors may be propagated on a lawn of DH5α-FT, DH5αF', DH5αF'IQ, JM101, or JM107 for plaque formation
Description
1.DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml)
2. pUC19 DNA (10 pg/ul): 1 vial, 50 µl
3.S.O.C. medium: 2 bottles, 6 ml each
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4C or at Room Temperature.
Industry Terms
Not Available to Industry
