Product Name
DH5α-T1R Competent Cells
Expression System
Multiple Expression System
Application
Bacteria Competent Cells
Genotype
F-φ80dlacZΔM15Δ(lacZYA-argF)U169 recA1 endA1 tonA hsdR17(rK-, mK+) phoA supE44 λ -thi-1 gyrA96 relA1
Screening Method
Blue/White Screening
Cloning Methylated DNA
No
Competent Cell Type
Chemically competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
yes
T1 Phage - Resistant (tonA)
yes
Transformation Efficiency
> 1 × 109
Transformation Efficiency Level
High Efficiency (> 109 cfu⁄µg)
Improves Plasmid Quality
yes
Feature
1. Cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
2. Reduced occurrence of unwanted recombination in cloned DNA (recA1)
3. Efficient transformation of unmethylated DNA from PCR applications (hsdR)
4. Blue/white color screening of recombinant clones carrying the alpha fragment of β-galactosidase (lacZΔM15)
Description
1. DH5α-T1R Competent Cells: 20 vials, 50 µl each ( total of 1 ml)
2. pUC19 DNA (10 pg/ul): 1 vial, 50 µl
3. S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature
Industry Terms
Not Available to Industry
