DMEM/F‑12 (1:1) with phenol red and L‑glutamine, without HEPES

Product Name

DMEM/F‑12 (1:1) with phenol red and L‑glutamine, without HEPES

Classification

Basal Medium

Format

Bottle(s)

Form

Liquid

Recommended Cell Line

Neurons, glial cells, endothelial cells, etc.

Storage Conditions

2–8°C, protect from light

Shipping Conditions

Ice Pack (Blue Ice)

Feature

Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO₂
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 290–330 mOsmol/kg
pH Range: 7.0–7.4
Raw Material Origin: Animal-origin free

Description

DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12, widely used to support the growth of various mammalian cells under low serum conditions. It is suitable for MDCK cells, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. DMEM provides elevated levels of amino acids and vitamins, while Ham's F-12 contributes increased concentrations of choline, inositol, putrescine, and selected amino acids, making this medium ideal for a range of cell types.
This product is prepared using Water for Injection (WFI).
With (+)
• L-glutamine
• Phenol red
Without (-)
• HEPES