Ham’s F‑12K (Kaighn’s modification) with phenol red and L‑glutamine

Product Name

Ham’s F‑12K (Kaighn’s modification) with phenol red and L‑glutamine

Classification

Basal Medium

Format

Bottle(s)

Form

Liquid

Recommended Cell Line

Neurons, glial cells, endothelial cells, etc.

Storage Conditions

2–8°C, protect from light

Shipping Conditions

Ice Pack (Blue Ice)

Feature

Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO₂
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 310–350 mOsmol/kg
pH Range: 7.5–7.9
Raw Material Origin: Animal-origin free

Description

Ham's F-12K Nutrient Mixture (Kaighn's Modification) is a modified formulation of Ham’s F-12 medium, specifically developed for the culture of primary human hepatocytes and certain rat and chicken liver cells under reduced-serum conditions. This medium contains putrescine, thymidine, hypoxanthine, zinc, elevated concentrations of all amino acids, and sodium pyruvate, which support the growth of specific cell types in low-serum environments.
However, when used under reduced-serum conditions, it is essential to optimize the serum concentration for each individual cell line.
This product is prepared using Water for Injection (WFI).
With (+)
• Phenol red
• L-glutamine