High‑glucose DMEM (ATCC formulation) with L‑glutamine, sodium pyruvate and 1.5 g/L sodium bicarbonate

Product Name

High‑glucose DMEM (ATCC formulation) with L‑glutamine, sodium pyruvate and 1.5 g/L sodium bicarbonate

Classification

Basal Medium

Format

Bottle(s)

Form

Liquid

Recommended Cell Line

Fibroblasts, smooth muscle cells, etc.

Storage Conditions

2–8°C, protect from light

Shipping Conditions

Ice Pack (Blue Ice)

Feature

Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO₂
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 260–320 mOsmol/kg
pH Range: 7.0–7.4
Raw Material Origin: Animal-origin free

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium suitable for the culture of many mammalian cell types, including HeLa, 293, Cos-7, and PC-12 cell lines, as well as primary fibroblasts, neurons, glial cells, human umbilical vein endothelial cells (HUVECs), and smooth muscle cells. Compared to MEM, DMEM contains four times the concentration of amino acids and vitamins, supporting enhanced cell growth and maintenance.
This product is prepared using Water for Injection (WFI).
With (+)
• 4.5 g/L D-glucose
• L-glutamine
• Phenol red
• 1.5 g/L Sodium pyruvate
Without (-)
• HEPES