Product Name
Insect 6 × His Expression and Purification Kit
Link To
Expression Related Kit
Expression System
Insect Expression System
Category
Protein expression kit
Application
Protein expression in the baculovirus system
Related Vector
pAcHLT-A,B,C Baculovirus Transfer Vector Set
pAcHLT-XylE Baculovirus Control Vector
Subclone Construction Method
Restriction Enzyme ⁄ MCS
Recombination Method
Homologous Recombination
Industry Terms
Not Available to Industry
Contents
1. Linearized Baculovirus DNA （2.5 µg）
2. pAcHLT-A,B,C Baculovirus Transfer Vector Set （20 µg each）
3. pAcHLT-XylE Baculovirus Control Vector (5.0 µg)
4. Thrombin Powder ( 20 mg (1000 U))
5. Thrombin Dilution Buffer ( 1 ml)
6. Protease Inhibitor Cocktail (lyophilized)
7. 1x Insect Cell Lysis Buffer （50 ml）
8. His Purification Resin （10 ml）
9. 6xHis Elution Buffer （40 ml）
10. 6xHis Wash Buffer （250 ml）
11. 3M Imidazole Solution 125 ml
12. Transfection Buffer A&B Set 5 ml each
13. Baculovirus Procedures & Methods Manual 1
Description
Purifications to greater than 90% homogeneity are achieved in a single step under native conditions
