pAcGHLT-A,B,C Baculovirus Transfer Vector Set pAcGHLT-XylE Baculovirus Control Vector
Recommend Cell Line
Sf9
Subclone Construction Method
Restriction Enzyme ⁄ MCS
Recombination Method
Homologous Recombination
Protein Tag
GST-tag, 6xHis-tag
Industry Terms
Not Available to Industry
Contents
1. Linearized Baculovirus DNA 2.5 µg 2. pAcGHLT-A,B,C Baculovirus Transfer Vector Set 20 µg each 3. pAcGHLT-XylE Baculovirus Control Vector 5.0 µg 4. Thrombin Powder 20 mg 20 mg (1000 U) 5. Thrombin Dilution Buffer 1 ml 6. Protease Inhibitor Cocktail lyophilized 7. 1x Insect Cell Lysis Buffer 50 ml 8. Glutathione Powder 62 mg 9. Glutathione Agarose Beads 10 ml 10. GST Elution Buffer 40 ml 11. 1x PBS Wash Buffer 375 ml 12. Transfection Buffer A&B Set 5 ml each 13. Baculovirus Procedures & Methods Manual 1
Description
Purifications to greater than 90% homogeneity are achieved in a single step under native conditions
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