Product Name
Insect GST Expression and Purification Kit
Link To
Expression Related Kit
Expression System
Insect Expression System
Category
Protein expression kit
Application
Protein expression in the baculovirus system
Related Vector
pAcGHLT-A,B,C Baculovirus Transfer Vector Set
pAcGHLT-XylE Baculovirus Control Vector
Subclone Construction Method
Restriction Enzyme ⁄ MCS
Recombination Method
Homologous Recombination
Protein Tag
GST-tag, 6xHis-tag
Industry Terms
Not Available to Industry
Contents
1. Linearized Baculovirus DNA 2.5 µg
2. pAcGHLT-A,B,C Baculovirus Transfer Vector Set 20 µg each
3. pAcGHLT-XylE Baculovirus Control Vector 5.0 µg
4. Thrombin Powder 20 mg 20 mg (1000 U)
5. Thrombin Dilution Buffer 1 ml
6. Protease Inhibitor Cocktail lyophilized
7. 1x Insect Cell Lysis Buffer 50 ml
8. Glutathione Powder 62 mg
9. Glutathione Agarose Beads 10 ml
10. GST Elution Buffer 40 ml
11. 1x PBS Wash Buffer 375 ml
12. Transfection Buffer A&B Set 5 ml each
13. Baculovirus Procedures & Methods Manual 1
Description
Purifications to greater than 90% homogeneity are achieved in a single step under native conditions
