Product Name
Mach1 T1 Chemically Competent E. coli
Expression System
Insect Expression System
Application
Bacteria Competent Cells
Screening Method
Blue/White Screening
Cloning Methylated DNA
No
Competent Cell Type
Chemically competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
yes
T1 Phage - Resistant (tonA)
yes
Transformation Efficiency
> 1 × 109
Transformation Efficiency Level
High Efficiency (> 109 cfu⁄µg)
Improves Plasmid Quality
yes
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Shipping Conditions
Dry Ice
Feature
1. Minimizes nonspecific recombination in cloned DNA (recA)
2. Offers resistance to T1 and T5 phage (tonA)
3. Yields cleaner preparations of DNA and better results in downstream applications due to the elimination of nonspecific digestion by Endonuclease I (endA1)
4. Provides efficient transformation of unmethylated DNA from PCR amplifications (hsdR)
5. Allows blue/white screening of recombinant clones (lacZΔM15)
Description
1. Mach1 Chemically Competent E. coli : 21 vials, 50 µl each
2. pUC19 DNA (10 pg/µl): 1 vial, 50 µl
3. S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Industry Terms
Not Available to Industry
