Expression System
Cell-free Expression System
Antibiotic Resistance
Ampicillin
Protein Tag
His (N terminal on backbone)
Mutation
The fluorescent protein was inserted between XhoI and EcoR1 of pBad/His B. There is an extra 'C' after XhoI to avoid the frameshift.
Cloning Sites
5'-XhoI,3'-EcoRI
Cloning Methods
Restriction Enzyme
Form
Plasmid sent in bacteria as agar stab
Recommend Cell Line
DH5alpha
Industry Terms
Not Available to Industry
