Product Name
pMAL-c6T Vector, 10 µg (50 µl)
Vector Category
Expression Vector
Expression System
Bacterial Expression System
Promoter
Strong tac promoter
Application
Non-T7 Expression, Target Protein Insolubility , Expression of Difficult Proteins
Antibiotic Resistance
Ampicillin
Protein Tag
N-terminal encoded His tag, TEV cleavage site
Recommend Cell Line
10 beta competent E. coli
Industry Terms
Not Available to Industry
Description
The pMAL-c6T Vector is designed to produce maltose-binding protein (MBP) fusions in the cytoplasm. The MBP has been engineered for tighter binding to amylose resin. The vector expresses an N-terminal his-tagged malE gene followed by a multiple cloning site containing a TEV protease recognition sequence, allowing MBP to be cleaved from the protein of interest after purification.
