Product Name
pTXB1 Expression Vector (200 µg/ml)
Vector Category
Expression Vector
Expression System
Bacterial Expression System
Application
Protein Expression in E. Coli, Protein Expression
Antibiotic Resistance
Ampicillin
Protein Tag
Recommended for use in intein-mediated protein ligation and C-terminal labeling
Cloning Sites
5'-Sapl, 3'-Spel
Cloning Methods
Restriction Enzyme
Recommend Cell Line
BL21(DE3) Competent E. coli
Industry Terms
Not Available to Industry
Description
pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.
