Product Name
pTYB21 Expression Vector (200 µg/ml)
Vector Category
Expression Vector
Expression System
Bacterial Expression System
Application
Protein Expression in E. Coli, Protein Expression
Antibiotic Resistance
Ampicillin
Recommend Cell Line
BL21(DE3) Competent E. coli , T7 Express Competent E. coli (High Efficiency)
Industry Terms
Not Available to Industry
Description
pTYB21 is an E. coli expression N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.
