Cloning Methods
BamH I, Rsr II, BssH II, Stu I, Sal I, Sac I, Xba I, Pst I ,Bbs I, Sma I, Xma I, Xho I, Nhe I, Nsi I, Sph I
Description
The transfer vector pUCDM has an identical expression cassette including a multiplication module as pFBDM. This expression cassette is flanked by a LoxP inverted repeat. Vector pUCDM contains a chloramphenicol resistance marker and a conditional R6Kγ origin of replication which makes its propagation dependent on the expression of the pir gene in the prokaryotic host.
