RPMI 1640 (ATCC formulation) containing phenol red, 4.5 g/L D‑glucose, 300 mg/L L‑glutamine, 1 mM sodium pyruvate and 10 mM HEPES

Product Name

RPMI 1640 (ATCC formulation) containing phenol red, 4.5 g/L D‑glucose, 300 mg/L L‑glutamine, 1 mM sodium pyruvate and 10 mM HEPES

Classification

Basal Medium

Format

Bottle(s)

Form

Liquid

Recommended Cell Line

Leukemia cells, PBMC, etc.

Storage Conditions

2–8°C, protect from light

Shipping Conditions

Ice Pack (Blue Ice)

Feature

Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO₂
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 260–305 mOsmol/kg
pH Range: 7.0–7.4
Raw Material Origin: Animal-origin free

Description

Named after the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A medium originally developed for the suspension and monolayer culture of human leukemia cells. It is also widely used for HeLa, Jurkat, MCF-7, PC12, peripheral blood mononuclear cells (PBMCs), astrocytes, and various cancer cells. RPMI 1640 is distinguished by its inclusion of reduced glutathione and high levels of vitamins, along with nutrients not found in EMEM or DMEM such as biotin, vitamin B12, para-aminobenzoic acid, and elevated concentrations of inositol and choline chloride.
RPMI 1640 Medium (ATCC Modification) is a modified formulation of standard RPMI 1640 medium. In this version, the glucose concentration has been increased from 2.0 g/L to 4.5 g/L, while the sodium bicarbonate concentration has been reduced from 2.0 g/L to 1.5 g/L.
This product is prepared using Water for Injection (WFI).
With (+)
• 4.5 g/L D-glucose
• Phenol red
• 1 mM Sodium pyruvate
• HEPES
• 300 mg/L L-glutamine