Product Name
RPMI 1640 with phenol red, L‑glutamine and HEPES
Classification
Basal Medium
Recommended Cell Line
Leukemia cells, PBMC, etc.
Storage Conditions
2–8°C, protect from light
Shipping Conditions
Ice Pack (Blue Ice)
Feature
Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO2
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 260–305 mOsmol/kg
pH Range: 7.0–7.4
Raw Material Origin: Animal-origin free
Description
Named after the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A medium originally developed for the suspension and monolayer culture of human leukemia cells. It is also widely used for HeLa, Jurkat, MCF-7, PC12, peripheral blood mononuclear cells (PBMCs), astrocytes, and various cancer cells. RPMI 1640 is distinguished by its inclusion of reduced glutathione and high levels of vitamins, along with nutrients not found in EMEM or DMEM such as biotin, vitamin B12, para-aminobenzoic acid, and elevated concentrations of inositol and choline chloride.
This product is prepared using Water for Injection (WFI).
With (+)
• L-glutamine
• Phenol red
• HEPES
