Product Name
TOP10 Chemically Competent E. coli, 20 reaction
Expression System
Multiple Expression System
Application
Bacteria Competent Cells
Screening Method
Blue/White Screening
Cloning Methylated DNA
Yes
Competent Cell Type
Chemically competent
F'episome (to make ssDNA)
Lacks F' Episome
High-throughput Compatibility
Not High-throughput Compatible
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
yes
T1 Phage - Resistant (tonA)
no
Transformation Efficiency
> 1 × 109
Transformation Efficiency Level
High Efficiency (> 109 cfu⁄µg)
Improves Plasmid Quality
yes
Shipping Conditions
Dry Ice
Feature
1. hsdR for efficient transformation of unmethylated DNA from PCR amplifications
2. mcrA for efficient transformation of methylated DNA from genomic preparations
3. lacZΔM15 for blue/white color screening of recombinant clones
4. endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
5. recA1 for reduced occurrence of nonspecific recombination in cloned DNA
Description
1.TOP10 Chemically Competent E. coli : 21 vials, 50 µl each
2.pUC19 DNA (10 pg/µl): 1 vial, 50 µl
3.S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
Industry Terms
Not Available to Industry
