Product Name
Williams’ Medium E with glycine and sodium pyruvate, without phenol red, L‑glutamine or HEPES
Classification
Basal Medium
Recommended Cell Line
Primary hepatocytes, etc.
Storage Conditions
2–8°C, protect from light
Shipping Conditions
Ice Pack (Blue Ice)
Feature
Serum Supplementation: 10% Fetal Bovine Serum (FBS)
Culture Conditions: 37 °C, 5–10% CO2
Sterility Grade: Sterile-filtered
Shelf Life: 12 months
Endotoxin Level: ≤ 1 EU/mL
Osmolality Range: 305–335 mOsmol/kg
pH Range: 7.0–7.6
Raw Material Origin: Animal-origin free
Description
Williams’ Medium E was developed by Williams and Gunn for long-term, low-serum culture of adult rat hepatocytes. It is also suitable for culturing various mammalian stem cells. This formulation includes 2000 mg/L glucose, 50 mg/L glycine, and 25 mg/L sodium pyruvate, and lacks phenol red, L-glutamine, and HEPES. Supplementation with 5–10% FBS is recommended. Due to 2.2 g/L sodium bicarbonate content, it should be used in a 5–10% CO2 environment.
